It means Wales’ four regions – Dragons, Cardiff Blues, Ospreys and Scarlets – now know their match details for the first three rounds of fixtures.
Due to the ongoing Covid-19 pandemic, the first three rounds of fixtures will be derbies to limit cross-border travel.
It means the competition’s four new South African sides won’t be travelling to Europe, for now at least.
The DHL Stormers and the Cell C Sharks will compete in the cup competition’s first game on Friday, April 23 and will be followed by Ulster’s derby match with Connacht and Zebre’s trip to Edinburgh.
On Saturday lunchtime, Benetton Rugby will host Glasgow Warriors to start off a run of four matches which will see the Vodacom Bulls take on the Emirates Lions, Ospreys taking on Cardiff Blues and Leinster hosting Munster in a rematch of their Guinness PRO14 Final on March 27.
Dragons and Scarlets will finish off Round 1 on Sunday with a lunch time kick-off (13:00) at Rodney Parade.
All games will be available live across the Guinness PRO14 network of broadcasters: Premier Sports, Super Sport, eir Sport and DAZN with S4C and TG4 showing selected live free-to-air matches, deferred coverage and highlights throughout the Rainbow Cup.
Full kick-off details for the opening rounds of the Guinness PRO14 Rainbow Cup are below.
The PRO14 say confirmation for rounds four to six will follow once they have received all necessary approvals and permissions from the relevant governments and health authorities for the South African teams to travel.
Guinness PRO14 Rainbow Cup fixture details for Welsh regions:
Round 1
Saturday, April 24
Ospreys v Cardiff Blues – KO 17:15
Liberty Stadium, Swansea | Host Broadcaster Premier Sports | Deferred Coverage: 20:35 S4C
Sunday, April 25
Dragons v Scarlets – KO 13:00
Rodney Parade, Newport | Host Broadcaster Premier Sports| Deferred Coverage: 16:00 S4C
Round 2
Saturday, May 8
Scarlets v Ospreys– KO 19:35
Parc y Scarlets, Llanelli | Host Broadcaster: Premier Sports | Deferred Coverage: S4C
Sunday, May 9
Cardiff Blues v Dragons – KO 13:00
Cardiff Arms Park, Cardiff | Host Broadcaster: Premier Sports | Deferred Coverage: S4C
Round 3
Saturday, May 15
Scarlets v Cardiff Blues – KO 15.00
Parc y Scarlets, Llanelli | Host Broadcaster: Premier Sports | Deferred Coverage: S4C
Dragons v Ospreys – KO 13:00
Rodney Parade, Newport | Host Broadcaster: Premier Sports | Deferred Coverage: S4C
Round 4: Weekend of May 29.
Round 5: Weekend of June 5.
Round 6: Weekend of June 12.
Final: Weekend of June 19.