They will assemble in camp with the squad on Monday 17 February to prepare for the sold-out match against Ireland at Principality Stadium on Saturday 22 February.
Ospreys centre Owen Watkin has been released from the squad following an ACL injury, sustained against France in the opening round of the championship, that requires surgery.
Saracens fullback Liam Williams has also been released following a knee injury sustained against France.
Rob Howley remains under contract with the WRU but will not be part of the Wales coaching team for the remaining three fixtures.
WALES SQUAD FOR 2025 GUINNESS SIX NATIONS
Forwards (21)
Keiron Assiratti (Cardiff Rugby– 12 caps)
James Botham (Cardiff Rugby – 18 caps)
Elliot Dee (Dragons – 53 caps)
Taulupe Faletau (Cardiff Rugby – 105 caps)
Dafydd Jenkins (Exeter Chiefs – 20 caps)
WillGriff John (Sale Sharks – 2 caps)
Evan Lloyd (Cardiff Rugby – 7 caps)
Kemsley Mathias (Scarlets – 5 caps)
Jac Morgan (Ospreys – 20 caps) captain
Sam Parry (Ospreys – 7 caps)
Taine Plumtree (Scarlets – 7 caps)
Tommy Reffell (Leicester Tigers – 24 caps)
Will Rowlands (Racing 92 – 38 caps)
Nicky Smith (Leicester Tigers – 51 caps)
Freddie Thomas (Gloucester Rugby – 3 caps)
Gareth Thomas (Ospreys – 37 caps)
Henry Thomas (Scarlets – 6 caps)
Christ Tshiunza (Exeter Chiefs – 15 caps)
Aaron Wainwright (Dragons – 54 caps)
Ben Warren (Ospreys – uncapped)
Teddy Williams (Cardiff Rugby – 3 caps)
Backs (16)
Josh Adams (Cardiff Rugby – 61 caps)
Gareth Anscombe (Gloucester Rugby – 39 caps)
Ellis Bevan (Cardiff Rugby – 6 caps)
Dan Edwards (Ospreys – 2 caps)
Jarrod Evans (Harlequins – 8 caps)
Josh Hathaway (Gloucester Rugby – 3 caps)
Eddie James (Scarlets – 4 caps)
Max Llewellyn (Gloucester Rugby – 5 caps)
Ellis Mee (Scarlets – uncapped )
Blair Murray (Scarlets – 5 caps)
Joe Roberts (Scarlets – 2 caps)
Tom Rogers (Scarlets – 7 caps)
Ben Thomas (Cardiff Rugby – 9 caps)
Nick Tompkins (Saracens – 40 caps)
Rhodri Williams (Dragons – 7 caps)
Tomos Williams (Gloucester Rugby – 61 caps)
