The Welsh side travel to Ireland on the back of a three-match winning streak to face the reigning champions in their own backyard.
Welsh international Bryonie King captains the side which sees two changes from the starting line-up that did the double over Edinburgh.
King is selected in a backrow including fellow Wales Test star Gwennan Hopkins and Catrin Stewart.
The Gwalia pack sees one change with Danyelle Dinapoli coming into the front row, alongside Maisie Davies and Molly Wakely.
Welsh international Alaw Pyrs partners Lily Terry in the Lightning engine room.
Wales full back Jenny Hesketh, who came off the bench in the win over Edinburgh, makes her first start for Gwalia Lightning.
Fellow Welsh international Caitlin Lewis and Courtney Greenway are selected on the wings and join Hesketh in the Lightning back three.
The centre partnership of Kelsie Webster and Anwen Owen, who impressed against Edinburgh are selected in midfield, with fly half Carys Hughes and Wales scrum half Sian Jones at half back.
Catrina Nicholas-McLaughlin, Gwalia Lightning Head coach, said: “We know that that Wolfhounds as the top of the table side will be a real challenge for us, but we travel to Ireland with real confidence.
“We have showed we can match any side in the Celtic Challenge and fully deserve to be at the top end of the table. Our performances have shown we can find a way and compete and play some really exciting and attacking rugby.
“Wolfhounds are a team full of experienced internationals, but this is the kind of game we need to find out where Gwalia Lightning are in our growth as a team. We are looking forward to what promises to be a real battle.”
Gwalia Lightning (v Wolfhounds)
Jenny Hesketh, Courtney Greenway, Kelsie Webster, Anwen Owen, Caitlin Lewis, Carys Hughes, Sian Jones; Maisie Davies, Molly Wakely, Danyelle Dinapoli, Lily Terry, Alaw Pyrs, Bryonie King (captain), Catrin Stewart, Gwennan Hopkins
Replacements: Molly Mae Crabb, Dali Hopkins, Cana Williams, Paige Jones, Lottie Buffery-Latham, Katie Bevans, Freya Bell, Carys Williams-Morris
